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collection buffer  (TaKaRa)


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    Structured Review

    TaKaRa collection buffer
    Collection Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart-seq+buffer/SMART-Seq+mRNA/pm42107045-296-12-18
    Average 99 stars, based on 174 article reviews
    collection buffer - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Lysis:

    Article Title: A Cross-Species Enhancer-AAV Toolkit for Cell Type-Specific Targeting Across the Basal Ganglia
    Article Snippet: Sample preparation for SMART-Seq was performed using the SMART-Seq v4 kit (Takara Cat#634894) as described previously. .. Single cells were sorted into 8-well strips containing SMART-Seq lysis buffer with RNase inhibitor (0.17 U/μL; Takara Cat# ST0764) and were immediately frozen on dry ice for storage at −80°C. ..

    Article Title: Protocol for purifying and sequencing cell subpopulations based on RNA FISH signal in planaria.
    Article Snippet: .. Load desired wells of a 96well skirted PCR plate with an appropriate collection buffer (e.g., SMART-Seq mRNA kit (Takara Bio) lysis buffer). ..

    Article Title: The External Globus Pallidus is a Basal Ganglia Output Hub with Action-Specific Circuits
    Article Snippet: .. DAPI+/GFP+, DAPI+/mCherry+, and DAPI+/GFP+/mCherry+ nuclei were sorted as single nuclei into 8-well strip tubes containing SMART-seq lysis buffer with RNase inhibitor (0.17 U/μL; Takara ST0764). ..

    Article Title: Technical and biological sources of noise confound multiplexed enhancer AAV screening
    Article Snippet: .. 800 single nuclei (40 nuclei per ALM/STR per enhancer) were sorted into 8-well strips containing SMART-Seq lysis buffer with RNase inhibitor (0.17 U/μL; Takara Cat# ST0764) on ice and were immediately frozen at −80 °C. .. SMART-Seq reagents (Takara Cat# 634894). were used for reverse transcription and cDNA amplification following the manufacturer’s protocol.

    Article Title: The External Globus Pallidus is a Basal Ganglia Output Hub with Action-Specific Circuits
    Article Snippet: .. Following nuclear isolation, individual nuclei were sorted into 8-well strips containing SMART-seq lysis buffer with RNAse inhibitor (0.17 U/μL; Takara Cat#ST0764), frozen on dry ice, stored at -80°C and then shipped to the Allen Institute on dry ice. ..

    Article Title: Regulatory T cells restrain IL-15-mediated cytotoxic and bystander T cell activity in mucosal tissue without compromising antigen-driven memory
    Article Snippet: .. Treg were sorted into directly into SMART-Seq v4 Ultra Low Input lysis buffer (Takara Bio USA) using the BD Symphony S6 and Flash frozen; upon thawing samples underwent reverse transcription followed by PCR ampliccation to generate full length ampliced cDNA. cDNA was then submitted for bulk RNA-seq performed by the Genomics Core at Benaroya Research Institute. .. Sequencing libraries were constructed using the NexteraXT DNA sample preparation kit with unique dual indexes (Illumina) to generate Illumina- compatible barcoded libraries.

    Article Title: The production of the chemokine CCL2 by corneal sensory neurons initiates anti-viral immunity at the cornea and trigeminal ganglion
    Article Snippet: Individual cells were sucked into the pipette using negative pressure, transferred to tubes containing 12μL of lysis buffer (Smart Seq V4, Takara Bio) and frozen at −80C. .. Individual cells were sucked into the pipette using negative pressure, transferred to tubes containing 12μL of lysis buffer (Smart Seq V4, Takara Bio) and frozen at −80C. .. Full-length cDNA was generated from single cells with the Takara SMART-Seq Single-cell kit (Takara: 634473) according to the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Protocol for purifying and sequencing cell subpopulations based on RNA FISH signal in planaria.
    Article Snippet: .. Load desired wells of a 96well skirted PCR plate with an appropriate collection buffer (e.g., SMART-Seq mRNA kit (Takara Bio) lysis buffer). ..

    Article Title: Regulatory T cells restrain IL-15-mediated cytotoxic and bystander T cell activity in mucosal tissue without compromising antigen-driven memory
    Article Snippet: .. Treg were sorted into directly into SMART-Seq v4 Ultra Low Input lysis buffer (Takara Bio USA) using the BD Symphony S6 and Flash frozen; upon thawing samples underwent reverse transcription followed by PCR ampliccation to generate full length ampliced cDNA. cDNA was then submitted for bulk RNA-seq performed by the Genomics Core at Benaroya Research Institute. .. Sequencing libraries were constructed using the NexteraXT DNA sample preparation kit with unique dual indexes (Illumina) to generate Illumina- compatible barcoded libraries.

    Stripping Membranes:

    Article Title: The External Globus Pallidus is a Basal Ganglia Output Hub with Action-Specific Circuits
    Article Snippet: .. DAPI+/GFP+, DAPI+/mCherry+, and DAPI+/GFP+/mCherry+ nuclei were sorted as single nuclei into 8-well strip tubes containing SMART-seq lysis buffer with RNase inhibitor (0.17 U/μL; Takara ST0764). ..

    Isolation:

    Article Title: The External Globus Pallidus is a Basal Ganglia Output Hub with Action-Specific Circuits
    Article Snippet: .. Following nuclear isolation, individual nuclei were sorted into 8-well strips containing SMART-seq lysis buffer with RNAse inhibitor (0.17 U/μL; Takara Cat#ST0764), frozen on dry ice, stored at -80°C and then shipped to the Allen Institute on dry ice. ..

    Reverse Transcription:

    Article Title: Regulatory T cells restrain IL-15-mediated cytotoxic and bystander T cell activity in mucosal tissue without compromising antigen-driven memory
    Article Snippet: .. Treg were sorted into directly into SMART-Seq v4 Ultra Low Input lysis buffer (Takara Bio USA) using the BD Symphony S6 and Flash frozen; upon thawing samples underwent reverse transcription followed by PCR ampliccation to generate full length ampliced cDNA. cDNA was then submitted for bulk RNA-seq performed by the Genomics Core at Benaroya Research Institute. .. Sequencing libraries were constructed using the NexteraXT DNA sample preparation kit with unique dual indexes (Illumina) to generate Illumina- compatible barcoded libraries.

    RNA Sequencing:

    Article Title: Regulatory T cells restrain IL-15-mediated cytotoxic and bystander T cell activity in mucosal tissue without compromising antigen-driven memory
    Article Snippet: .. Treg were sorted into directly into SMART-Seq v4 Ultra Low Input lysis buffer (Takara Bio USA) using the BD Symphony S6 and Flash frozen; upon thawing samples underwent reverse transcription followed by PCR ampliccation to generate full length ampliced cDNA. cDNA was then submitted for bulk RNA-seq performed by the Genomics Core at Benaroya Research Institute. .. Sequencing libraries were constructed using the NexteraXT DNA sample preparation kit with unique dual indexes (Illumina) to generate Illumina- compatible barcoded libraries.

    Transferring:

    Article Title: The production of the chemokine CCL2 by corneal sensory neurons initiates anti-viral immunity at the cornea and trigeminal ganglion
    Article Snippet: Individual cells were sucked into the pipette using negative pressure, transferred to tubes containing 12μL of lysis buffer (Smart Seq V4, Takara Bio) and frozen at −80C. .. Individual cells were sucked into the pipette using negative pressure, transferred to tubes containing 12μL of lysis buffer (Smart Seq V4, Takara Bio) and frozen at −80C. .. Full-length cDNA was generated from single cells with the Takara SMART-Seq Single-cell kit (Takara: 634473) according to the manufacturer’s instructions.



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